Novel Coronavirus RT-PCR Detection Kit COVID-19 Detection Kit Testing Kit

COVID-19Detection Kit RT-PCR

32tests/kit,48 tests/kit, 96 tests/kit

This product is intended for the rapid detection of 2019-nCOV in human throat swab or sputum samples. Coronavirus is a +ssRNA virus with envelope. Its diameter is about 80-120 nm. Its genetic material is the largest of all RNA viruses. It is an important pathogen of many livestock, pets, including human diseases, and can cause many kinds of acute and chronic diseases. According to Taxonomy- Ninth Report of the International Committee on Taxonomy o/'Viruses, the Coronaviridae is divided into three genera: a, and y. Among them, a Coronavirus (such as human Coronavirus 229E, human Coronavims NL63), p Coronavims (such as human Coronavinis HKU1, human Coronavirus OC43 and SARS) can cause disease of varying degree to human.

[PRINCIPLE OF DETECTION]

This product is a fluorescent probc-bascd Taqman RT-PCR assay system. Firstly, the RNA of 2019-nCov will be reverse transcribed into cDNA by reverse transcriptase, and then PCR amplification will be perfonned with cDNA as template. During amplification of the template, the Taqman probe will be degraded due Io the 5'-3^! polymerase activity and exonuclease activity ofTaq DNA polymerase, then the separation of fluorescent reporter and quencher enables the fluorescent signal to be detected by instrument. The ORHab gene of 2019-nCoV will be detected qualitatively by FAM channel, lhe N gene of 2019-nCoV will be detected qualitatively by JOE channel, the E gene of 2019-nCoV will be detected qualitatively by ROX channel, and the internal reference will be detected by CY5 channel